Construction and Molecular Analysis of Gene Transfer Systems Derived from Bovine Immunodeficiency Virus
نویسندگان
چکیده
منابع مشابه
Gene transfer systems derived from Visna virus: analysis of virus production and infectivity.
Efficient transfer of therapeutic genes into nondividing human cells can be accomplished by inserting the genes into lentiviruses and infecting the cells with the modified viruses. The most developed lentivirus gene transfer systems are based on HIV-1, but because of the widespread HIV epidemic, the use of HIV-based vectors for gene therapy may be associated with a safety risk. In an attempt to...
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HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results f...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2001
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.75.7.3371-3382.2001